Instructor: Dr. Andy Methven (Eastern Illinois University, Emeritus) Students will be introduced to the fleshy fungi (Ascomycetes and Basidiomycetes) that occur on the Highlands Plateau. Emphasis will be placed on the analysis of macro- and micro-morphological features in the identification of genera and species as well as the ecological role of fungi in the ecosystem. Additional topics may be added depending on student interest. The daily routine will consist of a morning lecture followed by a field trip until early afternoon. Collections will be examined and identified after returning from the field and an opportunity provided to assemble a collection of dried fleshy fungi. Microscopes and chemical reagents necessary for accurate species determinations will be available for use. The laboratory will be open in the evenings for additional study of collections. Pre-requisites: Introductory biology, ecology, or permission of instructor For more information, visit https://highlandsbiological.org/summer-2023-academic-courses/.

Instructors: Dr. Steph Jeffries (NC State Univesity), Dr. Alan Weakley (UNC-Chapel Hill), and Dr. Julie Tuttle (UNC-Chapel Hill) Check back later for a description and pre-requisites. For more information, visit https://highlandsbiological.org/summer-2023-academic-courses/.

Instructor: Dr. Bob Youker (Western Carolina University) In this lecture/lab course, students will learn how to prepare their field samples for brightfield and fluorescence microscopic examination. Students will also learn how microscopes generate images, the experimental conditions needed for optimal imaging of their specimens, and learn basic image processing techniques for analysis. The course will be a combination of lecture and hands on specimen preparation and imaging (lab). Students can either provide specimens from their field research, or work with the instructor to collect rotifers and other aquatic micro-invertebrates from the field to use in the lab portion.    Lab time will be centered on learning the parts and functions of modern brightfield and fluorescence (widefield & confocal) microscopes, preparing specimens (e.g., staining with fluorescent dyes), and acquiring images of their specimens for analysis. Students will learn to acquire images using a widefield fluorescence microscope and take a “field trip” to WCU to use the state-of-the-art Leica Stellaris 5 confocal microscope. We will also discuss some other modern approaches to fluorescence microscopy, such as electron and super-resolution microscopies.  Each student will prepare a final report that compares brightfield to fluorescence images taken of their samples. The student will explain in detail the features observed using both methodologies and the advantages and disadvantages of each method. The student will also give a short oral presentation on one microscopic technique not covered in the course and this topic will be selected from a list provided by the instructor. Pre-requisites: Introductory biology sequence For more information, visit https://highlandsbiological.org/summer-2023-academic-courses/.